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Science · Photosynthesis: places and materials

Does released oxygen come from CO₂? Trace photosynthesis by place and atom

Edited by: Hitoiki Arcade / Hitoiki Learning team · Published: · Editorial policy

Plants take in carbon dioxide and release oxygen during photosynthesis. It is tempting to imagine that removing C from CO₂ simply leaves O₂. Yet the oxygen atoms released as O₂ by green plants come from water.

Separate three questions: where the process occurs, where organic carbon comes from, and where released oxygen comes from. Changing the place or atom being asked about changes the answer.

First example: a chloroplast is a place; chlorophyll is a pigment

The first example asks for an organelle: a chloroplast. Its thylakoid membranes contain light-absorbing chlorophyll and other pigments. Connect the organelle with the substance inside it rather than treating their names as interchangeable.

Zoom from a leaf cell into a chloroplast. This locates the process but does not yet identify where the atoms in its products came from.

Second example: trace organic carbon back to CO₂

The second example asks which gas supplies carbon. Carbon dioxide supplies the carbon incorporated into organic compounds. Incorporating CO₂ carbon into organic molecules is called carbon fixation.

Write a labelled arrow: C in CO₂ → C in organic compounds. Light supplies energy, not carbon atoms. This arrow traces C only; it makes no claim that O in CO₂ becomes released O₂.

Third example: trace released O₂ back to water

The third example changes the target to oxygen atoms in released O₂. In the light-dependent reactions, electrons are extracted from water. This oxidation of water produces oxygen, so the answer is water. Draw a separate arrow: O in water → O in released O₂.

Isolating water oxidation gives 2 H₂O → O₂ + 4 H⁺ + 4 e⁻. H⁺ means a hydrogen ion; e⁻ means an electron. The two water molecules supply two O atoms for one O₂ molecule. Both sides have four H atoms, and the +4 and −4 charges on the right cancel. This is a half-reaction for water oxidation, not an equation for all of photosynthesis.

Balance totals without inventing an atom pathway

A familiar net equation is 6 CO₂ + 6 H₂O → C₆H₁₂O₆ + 6 O₂, with light energy required. It balances the total numbers of C, H and O atoms. Balancing alone does not establish the path taken by each atom.

Both CO₂ and H₂O contain O. Seeing that letter is insufficient to identify the source of released O₂; use the actual process of water oxidation. After the examples, explain three separate answers without looking: chloroplast for place, CO₂ for organic carbon, and water for the oxygen atoms in released O₂.

TRY & READ

Check your understanding with examples

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Example 1 · Biology: plants

Which organelle contains chlorophyll and carries out photosynthesis in plants?

  1. The Golgi apparatus
  2. A lysosome
  3. A chloroplast
  4. The nucleus
Read the answer and explanation

Answer: A chloroplast

Pigments occur in structures such as the chloroplast's thylakoid membranes.

Sources for this example

Example 2 · Biology: plants

Which gas supplies carbon for plant photosynthesis?

  1. Carbon dioxide
  2. Oxygen
  3. Nitrogen
  4. Carbon monoxide
Read the answer and explanation

Answer: Carbon dioxide

The carbon in photosynthetic organic compounds comes from carbon dioxide. Carbon fixation incorporates that carbon into organic molecules. Light supplies energy, while water contributes to other parts of the process.

Read reasoning and more detail

Separating an energy source from a carbon source explains why sunlight itself is not the carbon-containing ingredient asked for here.

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Example 3 · Biology: plants

The oxygen atoms released as O₂ during plant photosynthesis mainly come from what?

  1. Nitrogen
  2. Starch
  3. Water
  4. Carbon dioxide
Read the answer and explanation

Answer: Water

Water is oxidized during the light-dependent reactions, releasing oxygen.

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